4LRK

Bacterial Effector NleH2 Kinase Domain

4LRK の概要

• エントリーDOI: 10.2210/pdb4lrk/pdb
• 関連するPDBエントリー: 4LRJ
• 分子名称: Effector NleH2 (2 entities in total)
• 機能のキーワード: structural genomics, montreal-kingston bacterial structural genomics initiative, bsgi, kinase fold, bacterial effector kinase, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 74791.59

構造登録者

Cygler, M.,Grishin, A.M.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2013-07-19, 公開日: 2014-01-22, 最終更新日: 2024-02-28)

主引用文献

Grishin, A.M.,Cherney, M.,Anderson, D.H.,Phanse, S.,Babu, M.,Cygler, M.
NleH defines a new family of bacterial effector kinases.
Structure, 22:250-259, 2014

PubMed Abstract: Upon host cell infection, pathogenic Escherichia coli hijacks host cellular processes with the help of 20-60 secreted effector proteins that subvert cellular processes to create an environment conducive to bacterial survival. The NleH effector kinases manipulate the NF-κB pathway and prevent apoptosis. They show low sequence similarity to human regulatory kinases and contain two domains, the N-terminal, likely intrinsically unfolded, and a C-terminal kinase-like domain. We show that these effectors autophosphorylate on sites located predominantly in the N-terminal segment. The kinase domain displays a minimal kinase fold, but lacks an activation loop and the GHI subdomain. Nevertheless, all catalytically important residues are conserved. ATP binding proceeds with minimal structural rearrangements. The NleH structure is the first for the bacterial effector kinases family. NleHs and their homologous effector kinases form a new kinase family within the cluster of eukaryotic-like kinases that includes also Rio, Bud32, and KdoK families.

PubMed: 24373767
DOI: 10.1016/j.str.2013.11.006

実験手法

X-RAY DIFFRACTION (2.274 Å)