4LQ8

Rickettsia rickettsii cell surface antigen 4 (sca4) head domain (residues 21-360)

Summary for 4LQ8

• Entry DOI: 10.2210/pdb4lq8/pdb
• Related: 3h2u 3h2v 3smx 3vf0 3zzy 3zzz
• Descriptor: Sca-family protein (2 entities in total)
• Functional Keywords: cell adhesion
• Biological source: Rickettsia rickettsii
• Total number of polymer chains: 2
• Total formula weight: 75347.70

Authors

Lee, J.H.,Vonrhein, C.,Bricogne, G.,Izard, T. (deposition date: 2013-07-17, release date: 2013-08-28, Last modification date: 2024-02-28)

Primary citation

Lee, J.H.,Vonrhein, C.,Bricogne, G.,Izard, T.
Crystal structure of the N-terminal domains of the surface cell antigen 4 of Rickettsia.
Protein Sci., 22:1425-1431, 2013

PubMed Abstract: The obligate intracellular, gram-negative bacterium Rickettsia is the causative agent of spotted fevers and typhus in humans. Surface cell antigen (sca) proteins surround these bacteria. We recently reported the co-localization of one of these proteins, sca4, with vinculin in cells at sites of focal adhesions and demonstrated that two vinculin binding sites directed the sca4/vinculin interaction. Here we report the 2.2 Å crystal structure of the conserved N-terminal 38 kDa domain of sca4 from Rickettsia rickettsii. The structure reveals two subdomains. The first is an all-helical domain that is folded in a fashion similar to the dimeric assembly chaperone for rubisco, namely RbcX. The following and highly conserved β-strand domain lacks significant structural similarity with other known structures and to the best of our knowledge represents a new protein fold.

PubMed: 23904352
DOI: 10.1002/pro.2322

Experimental method

X-RAY DIFFRACTION (2.2 Å)