4LQ8

Rickettsia rickettsii cell surface antigen 4 (sca4) head domain (residues 21-360)

4LQ8 の概要

• エントリーDOI: 10.2210/pdb4lq8/pdb
• 関連するPDBエントリー: 3h2u 3h2v 3smx 3vf0 3zzy 3zzz
• 分子名称: Sca-family protein (2 entities in total)
• 機能のキーワード: cell adhesion
• 由来する生物種: Rickettsia rickettsii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75347.70

構造登録者

Lee, J.H.,Vonrhein, C.,Bricogne, G.,Izard, T. (登録日: 2013-07-17, 公開日: 2013-08-28, 最終更新日: 2024-02-28)

主引用文献

Lee, J.H.,Vonrhein, C.,Bricogne, G.,Izard, T.
Crystal structure of the N-terminal domains of the surface cell antigen 4 of Rickettsia.
Protein Sci., 22:1425-1431, 2013

PubMed Abstract: The obligate intracellular, gram-negative bacterium Rickettsia is the causative agent of spotted fevers and typhus in humans. Surface cell antigen (sca) proteins surround these bacteria. We recently reported the co-localization of one of these proteins, sca4, with vinculin in cells at sites of focal adhesions and demonstrated that two vinculin binding sites directed the sca4/vinculin interaction. Here we report the 2.2 Å crystal structure of the conserved N-terminal 38 kDa domain of sca4 from Rickettsia rickettsii. The structure reveals two subdomains. The first is an all-helical domain that is folded in a fashion similar to the dimeric assembly chaperone for rubisco, namely RbcX. The following and highly conserved β-strand domain lacks significant structural similarity with other known structures and to the best of our knowledge represents a new protein fold.

PubMed: 23904352
DOI: 10.1002/pro.2322

実験手法

X-RAY DIFFRACTION (2.2 Å)