4LP7

Crystal structure of the human metapneumovirus matrix protein

4LP7 の概要

• エントリーDOI: 10.2210/pdb4lp7/pdb
• 分子名称: Matrix protein M, CALCIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: twisted beta sandwich, viral matrix, lipid binding, calcium binding protein
• 由来する生物種: Human metapneumovirus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 110876.50

構造登録者

Leyrat, C.,Harlos, K.,Grimes, J.M. (登録日: 2013-07-15, 公開日: 2013-12-18, 最終更新日: 2023-09-20)

主引用文献

Leyrat, C.,Renner, M.,Harlos, K.,Huiskonen, J.T.,Grimes, J.M.
Structure and self-assembly of the calcium binding matrix protein of human metapneumovirus.
Structure, 22:136-148, 2014

PubMed Abstract: The matrix protein (M) of paramyxoviruses plays a key role in determining virion morphology by directing viral assembly and budding. Here, we report the crystal structure of the human metapneumovirus M at 2.8 Å resolution in its native dimeric state. The structure reveals the presence of a high-affinity Ca²⁺ binding site. Molecular dynamics simulations (MDS) predict a secondary lower-affinity site that correlates well with data from fluorescence-based thermal shift assays. By combining small-angle X-ray scattering with MDS and ensemble analysis, we captured the structure and dynamics of M in solution. Our analysis reveals a large positively charged patch on the protein surface that is involved in membrane interaction. Structural analysis of DOPC-induced polymerization of M into helical filaments using electron microscopy leads to a model of M self-assembly. The conservation of the Ca²⁺ binding sites suggests a role for calcium in the replication and morphogenesis of pneumoviruses.

PubMed: 24316400
DOI: 10.1016/j.str.2013.10.013

実験手法

X-RAY DIFFRACTION (2.83 Å)