4LLO

Structure of the eag domain-CNBHD complex of the mouse EAG1 channel

4LLO の概要

• エントリーDOI: 10.2210/pdb4llo/pdb
• 分子名称: Potassium voltage-gated channel subfamily H member 1 (3 entities in total)
• 機能のキーワード: voltage-gated potassium channel, transport protein
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cell membrane (By similarity); Multi-pass membrane protein: Q60603 Q60603
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 142592.38

構造登録者

Haitin, Y.,Carlson, A.E.,Zagotta, W.N. (登録日: 2013-07-09, 公開日: 2013-08-28, 最終更新日: 2024-10-09)

主引用文献

Haitin, Y.,Carlson, A.E.,Zagotta, W.N.
The structural mechanism of KCNH-channel regulation by the eag domain.
Nature, 501:444-448, 2013

PubMed Abstract: The KCNH voltage-dependent potassium channels (ether-à-go-go, EAG; EAG-related gene, ERG; EAG-like channels, ELK) are important regulators of cellular excitability and have key roles in diseases such as cardiac long QT syndrome type 2 (LQT2), epilepsy, schizophrenia and cancer. The intracellular domains of KCNH channels are structurally distinct from other voltage-gated channels. The amino-terminal region contains an eag domain, which is composed of a Per-Arnt-Sim (PAS) domain and a PAS-cap domain, whereas the carboxy-terminal region contains a cyclic nucleotide-binding homology domain (CNBHD), which is connected to the pore through a C-linker domain. Many disease-causing mutations localize to these specialized intracellular domains, which underlie the unique gating and regulation of KCNH channels. It has been suggested that the eag domain may regulate the channel by interacting with either the S4-S5 linker or the CNBHD. Here we present a 2 Å resolution crystal structure of the eag domain-CNBHD complex of the mouse EAG1 (also known as KCNH1) channel. It displays extensive interactions between the eag domain and the CNBHD, indicating that the regulatory mechanism of the eag domain primarily involves the CNBHD. Notably, the structure reveals that a number of LQT2 mutations at homologous positions in human ERG, in addition to cancer-associated mutations in EAG channels, localize to the eag domain-CNBHD interface. Furthermore, mutations at the interface produced marked effects on channel gating, demonstrating the important physiological role of the eag domain-CNBHD interaction. Our structure of the eag domain-CNBHD complex of mouse EAG1 provides unique insights into the physiological and pathophysiological mechanisms of KCNH channels.

PubMed: 23975098
DOI: 10.1038/nature12487

実験手法

X-RAY DIFFRACTION (1.995 Å)