4LKJ

The structure of hemagglutinin L226Q mutant (H3 numbering) from a avian-origin H7N9 influenza virus (A/Anhui/1/2013) in complex with avian receptor analog 3'SLNLN

Summary for 4LKJ

• Entry DOI: 10.2210/pdb4lkj/pdb
• Related: 4LKG 4LKH 4LKI 4LKK
• Related PRD ID: PRD_900067
• Descriptor: hemagglutinin, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• Functional Keywords: homotrimer, virus attachment, membrane fusion, viral protein
• Biological source: Influenza A virus; More
• Total number of polymer chains: 2
• Total formula weight: 54982.04

Authors

Shi, Y.,Zhang, W.,Wang, F.,Qi, J.,Song, H.,Wu, Y.,Gao, F.,Zhang, Y.,Fan, Z.,Gong, W.,Wang, D.,Shu, Y.,Wang, Y.,Yan, J.,Gao, G.F. (deposition date: 2013-07-07, release date: 2013-11-06, Last modification date: 2024-11-06)

Primary citation

Shi, Y.,Zhang, W.,Wang, F.,Qi, J.,Wu, Y.,Song, H.,Gao, F.,Bi, Y.,Zhang, Y.,Fan, Z.,Qin, C.,Sun, H.,Liu, J.,Haywood, J.,Liu, W.,Gong, W.,Wang, D.,Shu, Y.,Wang, Y.,Yan, J.,Gao, G.F.
Structures and receptor binding of hemagglutinins from human-infecting H7N9 influenza viruses
Science, 342:243-247, 2013

PubMed Abstract: An avian-origin human-infecting influenza (H7N9) virus was recently identified in China. We have evaluated the viral hemagglutinin (HA) receptor-binding properties of two human H7N9 isolates, A/Shanghai/1/2013 (SH-H7N9) (containing the avian-signature residue Gln(226)) and A/Anhui/1/2013 (AH-H7N9) (containing the mammalian-signature residue Leu(226)). We found that SH-H7N9 HA preferentially binds the avian receptor analog, whereas AH-H7N9 HA binds both avian and human receptor analogs. Furthermore, an AH-H7N9 mutant HA (Leu(226) → Gln) was found to exhibit dual receptor-binding property, indicating that other amino acid substitutions contribute to the receptor-binding switch. The structures of SH-H7N9 HA, AH-H7N9 HA, and its mutant in complex with either avian or human receptor analogs show how AH-H7N9 can bind human receptors while still retaining the avian receptor-binding property.

PubMed: 24009358
DOI: 10.1126/science.1242917

Experimental method

X-RAY DIFFRACTION (2.798 Å)