4LEL

Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCG-G on the Ribosome

これはPDB形式変換不可エントリーです。

4LEL の概要

• エントリーDOI: 10.2210/pdb4lel/pdb
• 分子名称: 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (59 entities in total)
• 機能のキーワード: protein biosynthesis, ribosomes, rna, trna, transfer rna, 30s, 70s, 16s, 23s, ribosomal subunit, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 112
• 化学式量合計: 4435746.02

構造登録者

Maehigashi, T.,Dunkle, J.A.,Dunham, C.M. (登録日: 2013-06-25, 公開日: 2014-08-06, 最終更新日: 2024-11-20)

主引用文献

Maehigashi, T.,Dunkle, J.A.,Miles, S.J.,Dunham, C.M.
Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.
Proc.Natl.Acad.Sci.USA, 111:12740-12745, 2014

PubMed Abstract: Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNA(SufA6) and N1-methylguanosine at position 37 (m(1)G37) modification-deficient anticodon stem loop(Pro), both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNA(SufA6) to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m(1)G37 modification of tRNA(Pro) also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.

PubMed: 25128388
DOI: 10.1073/pnas.1409436111

実験手法

X-RAY DIFFRACTION (3.90000024172 Å)