4LDM

Crystal Structure of an RNA-free VP40 Octameric Ring

4LDM の概要

• エントリーDOI: 10.2210/pdb4ldm/pdb
• 関連するPDBエントリー: 4LD8 4LDB 4LDD 4LDI
• 分子名称: Matrix protein VP40 (2 entities in total)
• 機能のキーワード: ebolavirus matrix protein, rna binding ring, viral transcription regulation, viral protein
• 由来する生物種: Ebola virus (ZEBOV)
• 細胞内の位置: Virion membrane; Peripheral membrane protein: Q05128
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17502.07

構造登録者

Bornholdt, Z.A.,Ableson, D.M.,Saphire, E.O. (登録日: 2013-06-24, 公開日: 2013-08-21, 最終更新日: 2024-02-28)

主引用文献

Bornholdt, Z.A.,Noda, T.,Abelson, D.M.,Halfmann, P.,Wood, M.R.,Kawaoka, Y.,Saphire, E.O.
Structural Rearrangement of Ebola Virus VP40 Begets Multiple Functions in the Virus Life Cycle.
Cell(Cambridge,Mass.), 154:763-774, 2013

PubMed Abstract: Proteins, particularly viral proteins, can be multifunctional, but the mechanisms behind multifunctionality are not fully understood. Here, we illustrate through multiple crystal structures, biochemistry, and cellular microscopy that VP40 rearranges into different structures, each with a distinct function required for the ebolavirus life cycle. A butterfly-shaped VP40 dimer traffics to the cellular membrane. Once there, electrostatic interactions trigger rearrangement of the polypeptide into a linear hexamer. These hexamers construct a multilayered, filamentous matrix structure that is critical for budding and resembles tomograms of authentic virions. A third structure of VP40, formed by a different rearrangement, is not involved in virus assembly but instead uniquely binds RNA to regulate viral transcription inside infected cells. These results provide a functional model for ebolavirus matrix assembly and the other roles of VP40 in the virus life cycle and demonstrate how a single wild-type, unmodified polypeptide can assemble into different structures for different functions.

PubMed: 23953110
DOI: 10.1016/j.cell.2013.07.015

実験手法

X-RAY DIFFRACTION (1.85 Å)