4L76

Ca2+-bound E212Q mutant MthK RCK domain

4L76 の概要

• エントリーDOI: 10.2210/pdb4l76/pdb
• 関連するPDBエントリー: 4L73 4L74 4L75
• 分子名称: Calcium-gated potassium channel MthK, CALCIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: rossmann fold, regulatory domain, calcium binding, membrane-associated, metal transport
• 由来する生物種: Methanothermobacter thermautotrophicus
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: O27564
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 161886.64

構造登録者

Smith, F.J.,Rothberg, B.S. (登録日: 2013-06-13, 公開日: 2013-10-23, 最終更新日: 2023-09-20)

主引用文献

Smith, F.J.,Pau, V.P.,Cingolani, G.,Rothberg, B.S.
Structural basis of allosteric interactions among Ca(2+)-binding sites in a K(+) channel RCK domain.
Nat Commun, 4:2621-2621, 2013

PubMed Abstract: Ligand binding sites within proteins can interact by allosteric mechanisms to modulate binding affinities and control protein function. Here we present crystal structures of the regulator of K+ conductance (RCK) domain from a K+ channel, MthK, which reveal the structural basis of allosteric coupling between two Ca2+ regulatory sites within the domain. Comparison of RCK domain crystal structures in a range of conformations and with different numbers of regulatory Ca2+ ions bound, combined with complementary electrophysiological analysis of channel gating, suggests chemical interactions that are important for modulation of ligand binding and subsequent channel opening.

PubMed: 24126388
DOI: 10.1038/ncomms3621

実験手法

X-RAY DIFFRACTION (2.992 Å)