4L5R

Crystal structure of p202 HIN1 in complex with 20-mer dsDNA

Summary for 4L5R

• Entry DOI: 10.2210/pdb4l5r/pdb
• Descriptor: Interferon-activable protein 202, 20-mer DNA, SODIUM ION, ... (4 entities in total)
• Functional Keywords: hin200, ob fold, dsdna binding domain, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Mus musculus (mouse); More
• Cellular location: Cytoplasm: Q9R002
• Total number of polymer chains: 3
• Total formula weight: 35026.02

Authors

Yin, Q.,Tian, Y.,Wu, H. (deposition date: 2013-06-11, release date: 2013-07-31, Last modification date: 2024-02-28)

Primary citation

Yin, Q.,Sester, D.P.,Tian, Y.,Hsiao, Y.S.,Lu, A.,Cridland, J.A.,Sagulenko, V.,Thygesen, S.J.,Choubey, D.,Hornung, V.,Walz, T.,Stacey, K.J.,Wu, H.
Molecular Mechanism for p202-Mediated Specific Inhibition of AIM2 Inflammasome Activation.
Cell Rep, 4:327-339, 2013

PubMed Abstract: Mouse p202 containing two hemopoietic expression, interferon inducibility, nuclear localization (HIN) domains antagonizes AIM2 inflammasome signaling and potentially modifies lupus susceptibility. We found that only HIN1 of p202 binds double-stranded DNA (dsDNA), while HIN2 forms a homotetramer. Crystal structures of HIN1 revealed that dsDNA is bound on face opposite the site used in AIM2 and IFI16. The structure of HIN2 revealed a dimer of dimers, the face analogous to the HIN1 dsDNA binding site being a dimerization interface. Electron microscopy imaging showed that HIN1 is flexibly linked to HIN2 in p202, and tetramerization provided enhanced avidity for dsDNA. Surprisingly, HIN2 of p202 interacts with the AIM HIN domain. We propose that this results in a spatial separation of the AIM2 pyrin domains, and indeed p202 prevented the dsDNA-dependent clustering of apoptosis-associated speck-like protein containing caspase recruitment domain (ASC) and AIM2 inflammasome activation. We hypothesize that while p202 was evolutionarily selected to limit AIM2-mediated inflammation in some mouse strains, the same mechanism contributes to increased interferon production and lupus susceptibility.

PubMed: 23850291
DOI: 10.1016/j.celrep.2013.06.024

Experimental method

X-RAY DIFFRACTION (1.873 Å)