4L5L

Crystal structure of 26 kDa GST of Clonorchis sinensis in P212121 symmetry

Summary for 4L5L

• Entry DOI: 10.2210/pdb4l5l/pdb
• Related: 3ISO 4L5O
• Descriptor: Putative glutathione transferase, GLUTATHIONE, ZINC ION, ... (5 entities in total)
• Functional Keywords: transferase, gsh binding
• Biological source: Clonorchis sinensis (oriental liver fluke)
• Total number of polymer chains: 2
• Total formula weight: 51267.12

Authors

Chung, Y.J.,Han, Y.H. (deposition date: 2013-06-11, release date: 2013-09-04, Last modification date: 2024-03-20)

Primary citation

Han, Y.H.,Hong, S.J.,Cheong, H.K.,Chung, Y.J.
Crystal structures of 26kDa Clonorchis sinensis glutathione S-transferase reveal zinc binding and putative metal binding.
Biochem.Biophys.Res.Commun., 438:457-461, 2013

PubMed Abstract: The crystal structures of CsGST in two different space groups revealed that Asp26 and His79 coordinate a zinc ion. In one space group, His46 of an adjacent molecule participates in the coordination within 2.0Å. In the other space group, Asp26, His79 and a water molecule coordinate a zinc ion. The CsGST-D26H structure showed that four histidine residues - His26 and His79 from one molecule and the same residues from a symmetry-related neighboring molecule - coordinate a zinc ion. The coordinated zinc ions are located between two molecules and mediate molecular contacts within the crystal.

PubMed: 23916611
DOI: 10.1016/j.bbrc.2013.07.102

Experimental method

X-RAY DIFFRACTION (2.2 Å)