4L38

Nitrite complex of TvNiR, low dose data set

4L38 の概要

• エントリーDOI: 10.2210/pdb4l38/pdb
• 関連するPDBエントリー: 4L3X 4L3Y 4L3Z
• 分子名称: Eight-heme nitrite reductase, HEME C, NITRITE ION, ... (8 entities in total)
• 機能のキーワード: hemes c, oxidoreductase
• 由来する生物種: Thioalkalivibrio nitratireducens
• 細胞内の位置: Periplasm: L0DSL2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 129088.89

構造登録者

Trofimov, A.A.,Polyakov, K.M.,Lazarenko, V.A.,Popov, A.N.,Tikhonova, T.V.,Tikhonov, A.V.,Popov, V.O. (登録日: 2013-06-05, 公開日: 2014-07-16, 最終更新日: 2025-11-12)

主引用文献

Trofimov, A.A.,Polyakov, K.M.,Lazarenko, V.A.,Popov, A.N.,Tikhonova, T.V.,Tikhonov, A.V.,Popov, V.O.
Structural study of the X-ray-induced enzymatic reaction of octahaem cytochrome C nitrite reductase.
Acta Crystallogr.,Sect.D, 71:1087-1094, 2015

PubMed Abstract: Octahaem cytochrome c nitrite reductase from the bacterium Thioalkalivibrio nitratireducens catalyzes the reduction of nitrite to ammonium and of sulfite to sulfide. The reducing properties of X-ray radiation and the high quality of the enzyme crystals allow study of the catalytic reaction of cytochrome c nitrite reductase directly in a crystal of the enzyme, with the reaction being induced by X-rays. Series of diffraction data sets with increasing absorbed dose were collected from crystals of the free form of the enzyme and its complexes with nitrite and sulfite. The corresponding structures revealed gradual changes associated with the reduction of the catalytic haems by X-rays. In the case of the nitrite complex the conversion of the nitrite ions bound in the active sites to NO species was observed, which is the beginning of the catalytic reaction. For the free form, an increase in the distance between the oxygen ligand bound to the catalytic haem and the iron ion of the haem took place. In the case of the sulfite complex no enzymatic reaction was detected, but there were changes in the arrangement of the active-site water molecules that were presumably associated with a change in the protonation state of the sulfite ions.

PubMed: 25945574
DOI: 10.1107/S1399004715003053

実験手法

X-RAY DIFFRACTION (1.8 Å)