4L1I

Crystal structure of EGFP-based Calcium Sensor CatchER complexed with Ca

4L1I の概要

• エントリーDOI: 10.2210/pdb4l1i/pdb
• 関連するPDBエントリー: 4L12 4L13
• 分子名称: EGFP-based Calcium Sensor CatchER, CALCIUM ION (3 entities in total)
• 機能のキーワード: chromophore, luminescence, fluorescent protein, calcium sensor, metal ions, gadolinium, calcium binding protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26221.47

構造登録者

Zhang, Y.,Weber, I.T. (登録日: 2013-06-03, 公開日: 2013-12-11, 最終更新日: 2024-11-06)

主引用文献

Zhang, Y.,Reddish, F.,Tang, S.,Zhuo, Y.,Wang, Y.F.,Yang, J.J.,Weber, I.T.
Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics.
Acta Crystallogr.,Sect.D, 69:2309-2319, 2013

PubMed Abstract: Calcium ions, which are important signaling molecules, can be detected in the endoplasmic reticulum by an engineered mutant of green fluorescent protein (GFP) designated CatchER with a fast off-rate. High resolution (1.78-1.20 Å) crystal structures were analyzed for CatchER in the apo form and in complexes with calcium or gadolinium to probe the binding site for metal ions. While CatchER exhibits a 1:1 binding stoichiometry in solution, two positions were observed for each of the metal ions bound within the hand-like site formed by the carboxylate side chains of the mutated residues S147E, S202D, Q204E, F223E and T225E that may be responsible for its fast kinetic properties. Comparison of the structures of CatchER, wild-type GFP and enhanced GFP confirmed that different conformations of Thr203 and Glu222 are associated with the two forms of Tyr66 of the chromophore which are responsible for the absorbance wavelengths of the different proteins. Calcium binding to CatchER may shift the equilibrium for conformational population of the Glu222 side chain and lead to further changes in its optical properties.

PubMed: 24311573
DOI: 10.1107/S0907444913021306

実験手法

X-RAY DIFFRACTION (1.2 Å)