4KZM

Crystal Structure of TR3 LBD S553A Mutant

4KZM の概要

• エントリーDOI: 10.2210/pdb4kzm/pdb
• 関連するPDBエントリー: 4JGV 4KZI 4KZJ
• 分子名称: Nuclear receptor subfamily 4 group A member 1, GLYCEROL (3 entities in total)
• 機能のキーワード: orphan nuclear receptor, transcription
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57734.89

構造登録者

Li, F.,Zhang, Q.,Li, A.,Tian, X.,Cai, Q.,Wang, W.,Wang, Y.,Chen, H.,Xing, Y.,Wu, Q.,Lin, T. (登録日: 2013-05-30, 公開日: 2013-12-18, 最終更新日: 2024-03-20)

主引用文献

Wang, W.J.,Wang, Y.,Chen, H.Z.,Xing, Y.Z.,Li, F.W.,Zhang, Q.,Zhou, B.,Zhang, H.K.,Zhang, J.,Bian, X.L.,Li, L.,Liu, Y.,Zhao, B.X.,Chen, Y.,Wu, R.,Li, A.Z.,Yao, L.M.,Chen, P.,Zhang, Y.,Tian, X.Y.,Beermann, F.,Wu, M.,Han, J.,Huang, P.Q.,Lin, T.,Wu, Q.
Orphan nuclear receptor TR3 acts in autophagic cell death via mitochondrial signaling pathway.
Nat.Chem.Biol., 10:133-140, 2014

PubMed Abstract: Autophagy is linked to cell death, yet the associated mechanisms are largely undercharacterized. We discovered that melanoma, which is generally resistant to drug-induced apoptosis, can undergo autophagic cell death with the participation of orphan nuclear receptor TR3. A sequence of molecular events leading to cellular demise is launched by a specific chemical compound, 1-(3,4,5-trihydroxyphenyl)nonan-1-one, newly acquired from screening a library of TR3-targeting compounds. The autophagic cascade comprises TR3 translocation to mitochondria through interaction with the mitochondrial outer membrane protein Nix, crossing into the mitochondrial inner membrane through Tom40 and Tom70 channel proteins, dissipation of mitochondrial membrane potential by the permeability transition pore complex ANT1-VDAC1 and induction of autophagy. This process leads to excessive mitochondria clearance and irreversible cell death. It implicates a new approach to melanoma therapy through activation of a mitochondrial signaling pathway that integrates a nuclear receptor with autophagy for cell death.

PubMed: 24316735
DOI: 10.1038/nchembio.1406

実験手法

X-RAY DIFFRACTION (2.3 Å)