4KZF
The mechanism of the amidases: The effect of the mutation E142L in the amidase from Geobacillus pallidus
Summary for 4KZF
Entry DOI | 10.2210/pdb4kzf/pdb |
Related | 2PLQ 4GYL 4GYN |
Descriptor | Aliphatic amidase, CHLORIDE ION (3 entities in total) |
Functional Keywords | active site, chloride ion, cysteine 166 oxidation, amidase, hydrolase |
Biological source | Bacillus sp. |
Total number of polymer chains | 1 |
Total formula weight | 38660.33 |
Authors | Weber, B.W.,Sewell, B.T.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R. (deposition date: 2013-05-29, release date: 2013-08-21, Last modification date: 2023-11-08) |
Primary citation | Weber, B.W.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R.,Venter, G.A.,Gumbart, J.C.,Sewell, B.T. The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning. J.Biol.Chem., 288:28514-28523, 2013 Cited by PubMed: 23946488DOI: 10.1074/jbc.M113.503284 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.85 Å) |
Structure validation
Download full validation report