4KZF

The mechanism of the amidases: The effect of the mutation E142L in the amidase from Geobacillus pallidus

Summary for 4KZF

• Entry DOI: 10.2210/pdb4kzf/pdb
• Related: 2PLQ 4GYL 4GYN
• Descriptor: Aliphatic amidase, CHLORIDE ION (3 entities in total)
• Functional Keywords: active site, chloride ion, cysteine 166 oxidation, amidase, hydrolase
• Biological source: Bacillus sp.
• Total number of polymer chains: 1
• Total formula weight: 38660.33

Authors

Weber, B.W.,Sewell, B.T.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R. (deposition date: 2013-05-29, release date: 2013-08-21, Last modification date: 2023-11-08)

• Primary citation: Weber, B.W.,Kimani, S.W.,Varsani, A.,Cowan, D.A.,Hunter, R.,Venter, G.A.,Gumbart, J.C.,Sewell, B.T.; The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning.; J.Biol.Chem., 288:28514-28523, 2013; PubMed: 23946488; DOI: 10.1074/jbc.M113.503284

Experimental method

X-RAY DIFFRACTION (1.85 Å)