4KZ2

Crystal Structure of phi29 pRNA 3WJ Core

4KZ2 の概要

• エントリーDOI: 10.2210/pdb4kz2/pdb
• 分子名称: phi29 pRNA 3WJ core RNA 18 mer, phi29 pRNA 3WJ core RNA 20 mer, phi29 pRNA 3WJ core RNA 16 mer, ... (5 entities in total)
• 機能のキーワード: phi29 dna packaging motor prna core element, rna
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 17335.09

構造登録者

Zhang, H.,Endrizzi, J.A.,Shu, Y.,Haque, F.,Sauter, C.,Guo, P.,Chi, Y.-I. (登録日: 2013-05-29, 公開日: 2013-10-16, 最終更新日: 2024-04-03)

主引用文献

Zhang, H.,Endrizzi, J.A.,Shu, Y.,Haque, F.,Sauter, C.,Shlyakhtenko, L.S.,Lyubchenko, Y.,Guo, P.,Chi, Y.I.
Crystal structure of 3WJ core revealing divalent ion-promoted thermostability and assembly of the Phi29 hexameric motor pRNA.
Rna, 19:1226-1237, 2013

PubMed Abstract: The bacteriophage phi29 DNA packaging motor, one of the strongest biological motors characterized to date, is geared by a packaging RNA (pRNA) ring. When assembled from three RNA fragments, its three-way junction (3WJ) motif is highly thermostable, is resistant to 8 M urea, and remains associated at extremely low concentrations in vitro and in vivo. To elucidate the structural basis for its unusual stability, we solved the crystal structure of this pRNA 3WJ motif at 3.05 Å. The structure revealed two divalent metal ions that coordinate 4 nt of the RNA fragments. Single-molecule fluorescence resonance energy transfer (smFRET) analysis confirmed a structural change of 3WJ upon addition of Mg²⁺. The reported pRNA 3WJ conformation is different from a previously published construct that lacks the metal coordination sites. The phi29 DNA packaging motor contains a dodecameric connector at the vertex of the procapsid, with a central pore for DNA translocation. This portal connector serves as the foothold for pRNA binding to procapsid. Subsequent modeling of a connector/pRNA complex suggests that the pRNA of the phi29 DNA packaging motor exists as a hexameric complex serving as a sheath over the connector. The model of hexameric pRNA on the connector agrees with AFM images of the phi29 pRNA hexamer acquired in air and matches all distance parameters obtained from cross-linking, complementary modification, and chemical modification interference.

PubMed: 23884902
DOI: 10.1261/rna.037077.112

実験手法

X-RAY DIFFRACTION (3.05 Å)