4KON

The structure of hemagglutinin from avian-origin H7N9 influenza virus in complex with human receptor analog 6'SLNLN (NeuAcα2-6Galβ1-4GlcNAcβ1-3Galβ1-4Glc)

4KON の概要

• エントリーDOI: 10.2210/pdb4kon/pdb
• 関連するPDBエントリー: 4KOL 4KOM
• 分子名称: Hemagglutinin HA1, Hemagglutinin HA2, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose, ... (5 entities in total)
• 機能のキーワード: homotrimer, virus attachment and membrane fusion, viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54820.94

構造登録者

Shi, Y.,Zhang, W.,Wang, F.,Qi, J.,Song, H.,Wu, Y.,Gao, F.,Zhang, Y.,Fan, Z.,Gong, W.,Wang, D.,Shu, Y.,Wang, Y.,Yan, J.,Gao, G.F. (登録日: 2013-05-12, 公開日: 2013-11-06, 最終更新日: 2024-11-20)

主引用文献

Shi, Y.,Zhang, W.,Wang, F.,Qi, J.,Wu, Y.,Song, H.,Gao, F.,Bi, Y.,Zhang, Y.,Fan, Z.,Qin, C.,Sun, H.,Liu, J.,Haywood, J.,Liu, W.,Gong, W.,Wang, D.,Shu, Y.,Wang, Y.,Yan, J.,Gao, G.F.
Structures and receptor binding of hemagglutinins from human-infecting H7N9 influenza viruses.
Science, 342:243-247, 2013

PubMed Abstract: An avian-origin human-infecting influenza (H7N9) virus was recently identified in China. We have evaluated the viral hemagglutinin (HA) receptor-binding properties of two human H7N9 isolates, A/Shanghai/1/2013 (SH-H7N9) (containing the avian-signature residue Gln(226)) and A/Anhui/1/2013 (AH-H7N9) (containing the mammalian-signature residue Leu(226)). We found that SH-H7N9 HA preferentially binds the avian receptor analog, whereas AH-H7N9 HA binds both avian and human receptor analogs. Furthermore, an AH-H7N9 mutant HA (Leu(226) → Gln) was found to exhibit dual receptor-binding property, indicating that other amino acid substitutions contribute to the receptor-binding switch. The structures of SH-H7N9 HA, AH-H7N9 HA, and its mutant in complex with either avian or human receptor analogs show how AH-H7N9 can bind human receptors while still retaining the avian receptor-binding property.

PubMed: 24009358
DOI: 10.1126/science.1242917

実験手法

X-RAY DIFFRACTION (2.6 Å)