4KMQ

1.9 Angstrom resolution crystal structure of uncharacterized protein lmo2446 from Listeria monocytogenes EGD-e

Summary for 4KMQ

• Entry DOI: 10.2210/pdb4kmq/pdb
• Descriptor: Lmo2446 protein, MAGNESIUM ION (3 entities in total)
• Functional Keywords: uncharacterized protein, virulence, pathogenesis, csgid, structural genomics, niaid, national institute of allergy and infectious diseases, center for structural genomics of infectious diseases, tim-barrel, unknown function
• Biological source: Listeria monocytogenes
• Total number of polymer chains: 1
• Total formula weight: 122714.67

Authors

Halavaty, A.S.,Minasov, G.,Filippova, E.V.,Dubrovska, I.,Winsor, J.,Shuvalova, L.,Peterson, S.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2013-05-08, release date: 2013-05-29, Last modification date: 2024-11-20)

Primary citation

Light, S.H.,Cahoon, L.A.,Halavaty, A.S.,Freitag, N.E.,Anderson, W.F.
Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis.
Nat Microbiol, 2:16202-16202, 2016

PubMed Abstract: Here we employ a 'systems structural biology' approach to functionally characterize an unconventional α-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.

PubMed: 27819654
DOI: 10.1038/nmicrobiol.2016.202

Experimental method

X-RAY DIFFRACTION (1.9 Å)