4KMD

Crystal structure of Sufud60-Gli1p

4KMD の概要

• エントリーDOI: 10.2210/pdb4kmd/pdb
• 関連するPDBエントリー: 4KM8 4KM9 4KMA 4KMH
• 分子名称: Sufu, Zinc finger protein GLI1, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: protein peptide complex, protein binding-transcription complex, protein binding/transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm : Q9UMX1; Cytoplasm . Isoform 2: Cytoplasm : P08151
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51768.91

構造登録者

Zhang, Y.,Qi, X.,Zhang, Z.,Wu, G. (登録日: 2013-05-08, 公開日: 2013-11-20, 最終更新日: 2023-11-08)

主引用文献

Zhang, Y.,Fu, L.,Qi, X.,Zhang, Z.,Xia, Y.,Jia, J.,Jiang, J.,Zhao, Y.,Wu, G.
Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci.
Nat Commun, 4:2608-2608, 2013

PubMed Abstract: Hedgehog (Hh) signalling regulates embryonic development and adult tissue homoeostasis. Mutations of its pathway components including Suppressor of Fused (Sufu) and Gli/Ci predispose to cancers and congenital anomalies. The Sufu-Gli protein complex occupies a central position in the vertebrate Hh signalling pathway, especially in mammals. Here structures of full-length human and Drosophila Sufu, the human Sufu-Gli complex, along with normal mode analysis and FRET measurement results, reveal that Sufu alternates between 'open' and 'closed' conformations. The 'closed' form of Sufu is stabilized by Gli binding and inhibited by Hh treatment, whereas the 'open' state of Sufu is promoted by Gli-dissociation and Hh signalling. Mutations of critical interface residues disrupt the Sufu-Gli complex and prevent Sufu from repressing Gli-mediated transcription, tethering Gli in the cytoplasm and protecting Gli from the 26S proteasome-mediated degradation. Our study thus provides mechanistic insight into the mutual recognition and regulation between Sufu and Gli/Ci.

PubMed: 24217340
DOI: 10.1038/ncomms3608

実験手法

X-RAY DIFFRACTION (1.7 Å)