4KL8

High-resolution structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air

4KL8 の概要

• エントリーDOI: 10.2210/pdb4kl8/pdb
• 関連するPDBエントリー: 1CC1 4KN9 4KO1 4KO2 4KO3 4KO4
• 分子名称: Periplasmic [NiFeSe] hydrogenase small subunit, Nickel-dependent hydrogenase large subunit, IRON/SULFUR CLUSTER, ... (10 entities in total)
• 機能のキーワード: o2-resistance, h2-cleavage and production, seleninate, oxidoreductase
• 由来する生物種: Desulfomicrobium baculatum; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 175687.56

構造登録者

Volbeda, A.,Cavazza, C.,Fontecilla-Camps, J.C. (登録日: 2013-05-07, 公開日: 2013-07-10, 最終更新日: 2023-09-20)

主引用文献

Volbeda, A.,Amara, P.,Iannello, M.,De Lacey, A.L.,Cavazza, C.,Fontecilla-Camps, J.C.
Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.
Chem.Commun.(Camb.), 49:7061-7063, 2013

PubMed Abstract: This study shows how the NiFeSe site of an anaerobically purified O2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.

PubMed: 23811828
DOI: 10.1039/c3cc43619e

実験手法

X-RAY DIFFRACTION (1.52 Å)