4KFV

Structural insight into Golgi membrane stacking by GRASP65 and GRASP55

4KFV の概要

• エントリーDOI: 10.2210/pdb4kfv/pdb
• 関連するPDBエントリー: 4KFW
• 分子名称: Golgi reassembly-stacking protein 1, ZINC ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: pdz domain, golgi stacking protein, golgi, signaling protein
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23151.77

構造登録者

Liu, X.,Hu, J. (登録日: 2013-04-28, 公開日: 2013-08-21, 最終更新日: 2024-03-20)

主引用文献

Feng, Y.,Yu, W.,Li, X.,Lin, S.,Zhou, Y.,Hu, J.,Liu, X.
Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins
J.Biol.Chem., 288:28418-28427, 2013

PubMed Abstract: The stacking of Golgi cisternae involves GRASP65 and GRASP55. The oligomerization of the N-terminal GRASP domain of these proteins, which consists of two tandem PDZ domains, is required to tether the Golgi membranes. However, the molecular basis for GRASP assembly is unclear. Here, we determined the crystal structures of the GRASP domain of GRASP65 and GRASP55. The structures reveal similar homotypic interactions: the GRASP domain forms a dimer in which the peptide-binding pockets of the two neighboring PDZ2 domains face each other, and the dimers are further connected by the C-terminal tail of one GRASP domain inserting into the binding pocket of the PDZ1 domain in another dimer. Biochemical analysis suggests that both types of contacts are relatively weak but are needed in combination for GRASP-mediated Golgi stacking. Our results unveil a novel mode of membrane tethering by GRASP proteins and provide insight into the mechanism of Golgi stacking.

PubMed: 23940043
DOI: 10.1074/jbc.M113.478024

実験手法

X-RAY DIFFRACTION (2.2 Å)