4KF7

Nup188(aa1-1160) from Myceliophthora thermophila

4KF7 の概要

• エントリーDOI: 10.2210/pdb4kf7/pdb
• 関連するPDBエントリー: 4KF8
• 分子名称: Nup188 (2 entities in total)
• 機能のキーワード: nucleoporin, structural protein
• 由来する生物種: Myceliophthora thermophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 128246.49

構造登録者

Schwartz, T.U.,Andersen, K.R. (登録日: 2013-04-26, 公開日: 2013-06-19, 最終更新日: 2024-02-28)

主引用文献

Andersen, K.R.,Onischenko, E.,Tang, J.H.,Kumar, P.,Chen, J.Z.,Ulrich, A.,Liphardt, J.T.,Weis, K.,Schwartz, T.U.
Scaffold nucleoporins Nup188 and Nup192 share structural and functional properties with nuclear transport receptors.
Elife, 2:e00745-e00745, 2013

PubMed Abstract: Nucleocytoplasmic transport is mediated by nuclear pore complexes (NPCs) embedded in the nuclear envelope. About 30 different proteins (nucleoporins, nups) arrange around a central eightfold rotational axis to build the modular NPC. Nup188 and Nup192 are related and evolutionary conserved, large nucleoporins that are part of the NPC scaffold. Here we determine the structure of Nup188. The protein folds into an extended stack of helices where an N-terminal 130 kDa segment forms an intricate closed ring, while the C-terminal region is a more regular, superhelical structure. Overall, the structure has distant similarity with flexible S-shaped nuclear transport receptors (NTRs). Intriguingly, like NTRs, both Nup188 and Nup192 specifically bind FG-repeats and are able to translocate through NPCs by facilitated diffusion. This blurs the existing dogma of a clear distinction between stationary nups and soluble NTRs and suggests an evolutionary relationship between the NPC and the soluble nuclear transport machinery. DOI:http://dx.doi.org/10.7554/eLife.00745.001.

PubMed: 23795296
DOI: 10.7554/eLife.00745

実験手法

X-RAY DIFFRACTION (2.65 Å)