4KDP

TcaR-ssDNA complex crystal structure reveals the novel ssDNA binding mechanism of the MarR family proteins

4KDP の概要

• エントリーDOI: 10.2210/pdb4kdp/pdb
• 分子名称: TcaR transcription regulator, DNA (5'-D(*CP*GP*CP*AP*GP*CP*GP*CP*GP*CP*AP*GP*CP*CP*CP*TP*A)-3'), 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: multiple drug resistance, ssdna binding, antibiotics, staphylococci, transcription-dna complex, transcription/dna
• 由来する生物種: Staphylococcus epidermidis
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 132947.17

構造登録者

Chang, Y.M.,Chen, C.K.-M.,Wang, A.H.-J. (登録日: 2013-04-25, 公開日: 2014-03-19, 最終更新日: 2023-09-20)

主引用文献

Chang, Y.M.,Ho, C.H.,Chen, C.K.,Maestre-Reyna, M.,Chang-Chien, M.W.,Wang, A.H.
TcaR-ssDNA complex crystal structure reveals new DNA binding mechanism of the MarR family proteins.
Nucleic Acids Res., 42:5314-5321, 2014

PubMed Abstract: The teicoplanin-associated locus regulator (TcaR) regulates gene expression of proteins on the intercellular adhesion (ica) locus involved in staphylococci poly-N-acetylglucosamine biosynthesis. The absence of TcaR increases poly-N-acetylglucosamine production and promotes biofilm formation. Until recently, the mechanism of multiple antibiotic resistance regulator family protein members, such as TcaR, was restricted to binding double-stranded DNA. However, we recently found that TcaR strongly interacts with single-stranded DNA, which is a new role for this family of proteins. In this study, we report Staphylococcus epidermidis TcaR-single-stranded DNA complex structures. Our model suggests that TcaR and single-stranded DNA form a 61-symmetry polymer composed of TcaR dimers with single-stranded DNA that wraps outside the polymer and 12 nt per TcaR dimer. Single-stranded DNA binding to TcaR involves a large conformational change at the DNA binding lobe. Several point mutations involving the single-stranded DNA binding surface validate interactions between single-stranded DNA and TcaR. Our results extend the novel role of multiple antibiotic resistance regulator family proteins in staphylococci.

PubMed: 24531929
DOI: 10.1093/nar/gku128

実験手法

X-RAY DIFFRACTION (3.6 Å)