4KDL

Crystal structure of p97/VCP N in complex with OTU1 UBXL

4KDL の概要

• エントリーDOI: 10.2210/pdb4kdl/pdb
• 関連するPDBエントリー: 4KDI
• 分子名称: Transitional endoplasmic reticulum ATPase, Ubiquitin thioesterase OTU1 (3 entities in total)
• 機能のキーワード: aaa atpase, ubx-like domain, protein binding, protein-protein complex, beta-barrel, beta-grasp, signaling protein-hydrolase complex, signaling protein/hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, cytosol: P55072; Cytoplasm: P43558
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31499.99

構造登録者

Kim, S.J.,Kim, E.E. (登録日: 2013-04-25, 公開日: 2014-03-19, 最終更新日: 2023-09-20)

主引用文献

Kim, S.J.,Cho, J.,Song, E.J.,Kim, S.J.,Kim, H.M.,Lee, K.E.,Suh, S.W.,Kim, E.E.
Structural Basis for Ovarian Tumor Domain-containing Protein 1 (OTU1) Binding to p97/Valosin-containing Protein (VCP).
J.Biol.Chem., 289:12264-12274, 2014

PubMed Abstract: Valosin-containing protein (VCP), also known as p97, is an AAA(+) ATPase that plays an essential role in a broad array of cellular processes including the endoplasmic reticulum-associated degradation (ERAD) pathway. Recently, ERAD-specific deubiquitinating enzymes have been reported to be physically associated with VCP, although the exact mechanism is not yet clear. Among these enzymes is ovarian tumor domain-containing protein 1 (OTU1). Here, we report the structural basis for interaction between VCP and OTU1. The crystal structure of the ubiquitin regulatory X-like (UBXL) domain of OTU1 (UBXLOTU1) complexed to the N-terminal domain of VCP (NVCP) at 1.8-Å resolution reveals that UBXLOTU1 adopts a ubiquitin-like fold and binds at the interface of two subdomains of NVCP using the (39)GYPP(42) loop of UBXLOTU1 with the two prolines in cis- and trans-configurations, respectively. A mutagenesis study shows that this loop is not only critical for the interaction with VCP but also for its role in the ERAD pathway. Negative staining EM shows that one molecule of OTU1 binds to one VCP hexamer, and isothermal titration calorimetry suggests that the two proteins bind with a KD of 0.71 μM. Analytical size exclusion chromatography and isothermal titration calorimetry demonstrates that OTU1 can bind VCP in both the presence and absence of a heterodimer formed by ubiquitin fusion degradation protein 1 and nuclear localization protein 4.

PubMed: 24610782
DOI: 10.1074/jbc.M113.523936

実験手法

X-RAY DIFFRACTION (1.81 Å)