4K9F

Neutron structure of Perdeuterated Rubredoxin refined against 1.75 resolution data collected on the new IMAGINE instrument at HFIR, ORNL

4K9F の概要

• エントリーDOI: 10.2210/pdb4k9f/pdb
• 関連するPDBエントリー: 1VCX 3KYX 3RYG 3RZT 3SS2 4AR3
• 分子名称: Rubredoxin, FE (III) ION (3 entities in total)
• 機能のキーワード: iron, metal-binding, transport, electron transport
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6087.57

構造登録者

Munshi, P.,Meilleur, F.,Myles, D. (登録日: 2013-04-19, 公開日: 2013-12-04, 最終更新日: 2023-09-20)

主引用文献

Meilleur, F.,Munshi, P.,Robertson, L.,Stoica, A.D.,Crow, L.,Kovalevsky, A.,Koritsanszky, T.,Chakoumakos, B.C.,Blessing, R.,Myles, D.A.
The IMAGINE instrument: first neutron protein structure and new capabilities for neutron macromolecular crystallography.
Acta Crystallogr.,Sect.D, 69:2157-2160, 2013

PubMed Abstract: The first high-resolution neutron protein structure of perdeuterated rubredoxin from Pyrococcus furiosus (PfRd) determined using the new IMAGINE macromolecular neutron crystallography instrument at the Oak Ridge National Laboratory is reported. Neutron diffraction data extending to 1.65 Å resolution were collected from a relatively small 0.7 mm(3) PfRd crystal using 2.5 d (60 h) of beam time. The refined structure contains 371 out of 391, or 95%, of the D atoms of the protein and 58 solvent molecules. The IMAGINE instrument is designed to provide neutron data at or near atomic resolution (1.5 Å) from crystals with volume <1.0 mm(3) and with unit-cell edges <100 Å. Beamline features include novel elliptical focusing mirrors that deliver neutrons into a 2.0 × 3.2 mm focal spot at the sample position with full-width vertical and horizontal divergences of 0.5 and 0.6°, respectively. Variable short- and long-wavelength cutoff optics provide automated exchange between multiple-wavelength configurations (λmin = 2.0, 2.8, 3.3 Å to λmax = 3.0, 4.0, 4.5, ∼20 Å). These optics produce a more than 20-fold increase in the flux density at the sample and should help to enable more routine collection of high-resolution data from submillimetre-cubed crystals. Notably, the crystal used to collect these PfRd data was 5-10 times smaller than those previously reported.

PubMed: 24100333
DOI: 10.1107/S0907444913019604

実験手法

NEUTRON DIFFRACTION (1.75 Å)