4K7S

Crystal structure of Zn2-hUb (human ubiquitin) adduct from a solution 35 mM zinc acetate/1.3 mM hUb

Summary for 4K7S

• Entry DOI: 10.2210/pdb4k7s/pdb
• Related: 1UBQ 3EEC 3EFU 3EHV 3N30 3N32 4K7U 4K7W
• Descriptor: ubiquitin, ZINC ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: ligase, zn adduct
• Biological source: Homo sapiens (human)
• Cellular location: Ubiquitin: Cytoplasm : P0CG48
• Total number of polymer chains: 3
• Total formula weight: 26365.17

Authors

Fermani, S.,Falini, G.,Calvaresi, M.,Bottoni, A.,Arnesano, F.,Natile, G. (deposition date: 2013-04-17, release date: 2013-05-08, Last modification date: 2023-09-20)

Primary citation

Fermani, S.,Falini, G.,Calvaresi, M.,Bottoni, A.,Calo, V.,Mangini, V.,Arnesano, F.,Natile, G.
Conformational selection of ubiquitin quaternary structures driven by zinc ions.
Chemistry, 19:15480-15484, 2013

PubMed Abstract: Zinc ions bridging two ubiquitin molecules (with His68 at the interface) contribute to select a subset of conformers from the noncovalent dimer ensemble, thus restricting quaternary structure dynamics, which hampers apo-protein crystallization. The type of selected conformer is shown to determine the crystal packing, which varies from orthorhombic to cubic symmetry.

PubMed: 24123543
DOI: 10.1002/chem.201302229

Experimental method

X-RAY DIFFRACTION (1.76 Å)