4K7S

Crystal structure of Zn2-hUb (human ubiquitin) adduct from a solution 35 mM zinc acetate/1.3 mM hUb

4K7S の概要

• エントリーDOI: 10.2210/pdb4k7s/pdb
• 関連するPDBエントリー: 1UBQ 3EEC 3EFU 3EHV 3N30 3N32 4K7U 4K7W
• 分子名称: ubiquitin, ZINC ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: ligase, zn adduct
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Ubiquitin: Cytoplasm : P0CG48
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 26365.17

構造登録者

Fermani, S.,Falini, G.,Calvaresi, M.,Bottoni, A.,Arnesano, F.,Natile, G. (登録日: 2013-04-17, 公開日: 2013-05-08, 最終更新日: 2023-09-20)

主引用文献

Fermani, S.,Falini, G.,Calvaresi, M.,Bottoni, A.,Calo, V.,Mangini, V.,Arnesano, F.,Natile, G.
Conformational selection of ubiquitin quaternary structures driven by zinc ions.
Chemistry, 19:15480-15484, 2013

PubMed Abstract: Zinc ions bridging two ubiquitin molecules (with His68 at the interface) contribute to select a subset of conformers from the noncovalent dimer ensemble, thus restricting quaternary structure dynamics, which hampers apo-protein crystallization. The type of selected conformer is shown to determine the crystal packing, which varies from orthorhombic to cubic symmetry.

PubMed: 24123543
DOI: 10.1002/chem.201302229

実験手法

X-RAY DIFFRACTION (1.76 Å)