4JUG

Crystal structure of 1918 pandemic influenza virus hemagglutinin mutant D225G

4JUG の概要

• エントリーDOI: 10.2210/pdb4jug/pdb
• 関連するPDBエントリー: 4JTV 4JTX 4JU0 4JUH 4JUJ
• 分子名称: Hemagglutinin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: virus attachment, membrane fusion, viral protein
• 由来する生物種: Influenza A virus; 詳細
• 細胞内の位置: Virion membrane; Single-pass type I membrane protein (Potential): Q9WFX3 Q9WFX3
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 334682.68

構造登録者

Zhang, W.,Shi, Y.,Qi, J.,Gao, F.,Li, Q.,Fan, Z.,Yan, J.,Gao, G.F. (登録日: 2013-03-24, 公開日: 2013-05-01, 最終更新日: 2023-11-08)

主引用文献

Zhang, W.,Shi, Y.,Qi, J.,Gao, F.,Li, Q.,Fan, Z.,Yan, J.,Gao, G.F.
Molecular basis of the receptor binding specificity switch of the hemagglutinins from both the 1918 and 2009 pandemic influenza A viruses by a D225G substitution
J.Virol., 87:5949-5958, 2013

PubMed Abstract: Influenza A virus uses sialic acids as cell entry receptors, and there are two main receptor forms, α2,6 linkage or α2,3 linkage to galactose, that determine virus host ranges (mammalian or avian). The receptor binding hemagglutinins (HAs) of both 1918 and 2009 pandemic H1N1 (18H1 and 09H1, respectively) influenza A viruses preferentially bind to the human α2,6 linkage receptor. A single D225G mutation in both H1s switches receptor binding specificity from α2,6 linkage binding to dual receptor binding. However, the molecular basis for this specificity switch is not fully understood. Here, we show via H1-ligand complex structures that the D225G substitution results in a loss of a salt bridge between amino acids D225 and K222, enabling the key residue Q226 to interact with the avian receptor, thereby obtaining dual receptor binding. This is further confirmed by a D225E mutant that retains human receptor binding specificity with the salt bridge intact.

PubMed: 23514882
DOI: 10.1128/JVI.00545-13

実験手法

X-RAY DIFFRACTION (2.7 Å)