4JSO

The X-ray crystal structure of a thermophilic cellobiose binding protein bound with laminaripentaose

Summary for 4JSO

• Entry DOI: 10.2210/pdb4jso/pdb
• Related: 2O7I 3I5O 4JSD
• Descriptor: Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein, beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: periplasmic binding protein, thermophilic protein, cellulose, sugar binding protein, cellopentaose, laminaribiose, laminarpentaose binding protein
• Biological source: Thermotoga maritima
• Total number of polymer chains: 1
• Total formula weight: 68278.88

Authors

Munshi, P.,Cuneo, M.J. (deposition date: 2013-03-22, release date: 2013-10-09, Last modification date: 2023-09-20)

Primary citation

Munshi, P.,Stanley, C.B.,Ghimire-Rijal, S.,Lu, X.,Myles, D.A.,Cuneo, M.J.
Molecular details of ligand selectivity determinants in a promiscuous beta-glucan periplasmic binding protein.
Bmc Struct.Biol., 13:18-18, 2013

PubMed Abstract: Members of the periplasmic binding protein (PBP) superfamily utilize a highly conserved inter-domain ligand binding site that adapts to specifically bind a chemically diverse range of ligands. This paradigm of PBP ligand binding specificity was recently altered when the structure of the Thermotoga maritima cellobiose-binding protein (tmCBP) was solved. The tmCBP binding site is bipartite, comprising a canonical solvent-excluded region (subsite one), adjacent to a solvent-filled cavity (subsite two) where specific and semi-specific ligand recognition occur, respectively.

PubMed: 24090243
DOI: 10.1186/1472-6807-13-18

Experimental method

X-RAY DIFFRACTION (2.07 Å)