4JO9

Crystal structure of the human Nup49CCS2+3* Nup57CCS3* complex 1:2 stoichiometry

4JO9 の概要

• エントリーDOI: 10.2210/pdb4jo9/pdb
• 関連するPDBエントリー: 4JNU 4JNV 4JO7 4JQ5
• 分子名称: Nucleoporin p54, Nucleoporin p58/p45 (3 entities in total)
• 機能のキーワード: nucleocytoplasmic transport, transport protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus, nuclear pore complex : Q7Z3B4 Q9BVL2
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 19654.40

構造登録者

Stuwe, T.,Bley, C.J.,Mayo, D.J.,Hoelz, A. (登録日: 2013-03-18, 公開日: 2014-09-17, 最終更新日: 2024-02-28)

主引用文献

Stuwe, T.,Bley, C.J.,Thierbach, K.,Petrovic, S.,Schilbach, S.,Mayo, D.J.,Perriches, T.,Rundlet, E.J.,Jeon, Y.E.,Collins, L.N.,Huber, F.M.,Lin, D.H.,Paduch, M.,Koide, A.,Lu, V.,Fischer, J.,Hurt, E.,Koide, S.,Kossiakoff, A.A.,Hoelz, A.
Architecture of the fungal nuclear pore inner ring complex.
Science, 350:56-64, 2015

PubMed Abstract: The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary analyses of the ~425-kilodalton inner ring complex (IRC), which forms the central transport channel and diffusion barrier of the NPC, revealing its interaction network and equimolar stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96. The CNT•Nic96 structure reveals that Nic96 functions as an assembly sensor that recognizes the three-dimensional architecture of the CNT, thereby mediating the incorporation of a defined CNT state into the NPC. We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation.

PubMed: 26316600
DOI: 10.1126/science.aac9176

実験手法

X-RAY DIFFRACTION (2.499 Å)