4JO3
Crystal structure of rabbit mAb R20 Fab in complex with V3 C-terminus of HIV-1 Consensus B gp120
Summary for 4JO3
| Entry DOI | 10.2210/pdb4jo3/pdb |
| Related | 4JO1 4JO2 4JO4 |
| Descriptor | monoclonal anti-HIV-1 gp120 V3 antibody R20 light chain, monoclonal anti-HIV-1 gp120 V3 antibody R20 heavy chain, gp120, ... (5 entities in total) |
| Functional Keywords | ig, antibody, immune system-viral protein complex, immune system/viral protein |
| Biological source | Oryctolagus cuniculus (rabbit) More |
| Cellular location | Virion membrane : Q9YY05 |
| Total number of polymer chains | 6 |
| Total formula weight | 96423.12 |
| Authors | Pan, R.M.,Kong, X.P. (deposition date: 2013-03-16, release date: 2013-07-31, Last modification date: 2024-11-27) |
| Primary citation | Pan, R.,Sampson, J.M.,Chen, Y.,Vaine, M.,Wang, S.,Lu, S.,Kong, X.P. Rabbit Anti-HIV-1 Monoclonal Antibodies Raised by Immunization Can Mimic the Antigen-Binding Modes of Antibodies Derived from HIV-1-Infected Humans. J.Virol., 87:10221-10231, 2013 Cited by PubMed Abstract: The rabbit is a commonly used animal model in studying antibody responses in HIV/AIDS vaccine development. However, no rabbit monoclonal antibodies (MAbs) have been developed previously to study the epitope-specific antibody responses against HIV-1 envelope (Env) glycoproteins, and little is known about how the rabbit immune system can mimic the human immune system in eliciting such antibodies. Here we present structural analyses of two rabbit MAbs, R56 and R20, against the third variable region (V3) of HIV-1 gp120. R56 recognizes the well-studied immunogenic region in the V3 crown, while R20 targets a less-studied region at the C terminus of V3. By comparison of the Fab/epitope complex structures of these two antibodies raised by immunization with that of the corresponding human antibodies derived from patients chronically infected with HIV-1, we found that rabbit antibodies can recognize immunogenic regions of gp120 and mimic the binding modes of human antibodies. This result can provide new insight into the use of the rabbit as an animal model in AIDS vaccine development. PubMed: 23864637DOI: 10.1128/JVI.00843-13 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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