4JO2

Crystal structure of rabbit mAb R56 Fab in complex with V3 crown of HIV-1 Consensus A gp120

4JO2 の概要

• エントリーDOI: 10.2210/pdb4jo2/pdb
• 関連するPDBエントリー: 4JO1 4JO3 4JO4
• 分子名称: monoclonal anti-HIV-1 gp120 V3 antibody R56 light chain, monoclonal anti-HIV-1 gp120 V3 antibody R56 heavy chain, gp120, ... (5 entities in total)
• 機能のキーワード: ig, antibody, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• 細胞内の位置: Virion membrane : Q9YXH5
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94475.04

構造登録者

Pan, R.M.,Kong, X.P. (登録日: 2013-03-16, 公開日: 2013-07-31, 最終更新日: 2017-11-15)

主引用文献

Pan, R.,Sampson, J.M.,Chen, Y.,Vaine, M.,Wang, S.,Lu, S.,Kong, X.P.
Rabbit Anti-HIV-1 Monoclonal Antibodies Raised by Immunization Can Mimic the Antigen-Binding Modes of Antibodies Derived from HIV-1-Infected Humans.
J.Virol., 87:10221-10231, 2013

PubMed Abstract: The rabbit is a commonly used animal model in studying antibody responses in HIV/AIDS vaccine development. However, no rabbit monoclonal antibodies (MAbs) have been developed previously to study the epitope-specific antibody responses against HIV-1 envelope (Env) glycoproteins, and little is known about how the rabbit immune system can mimic the human immune system in eliciting such antibodies. Here we present structural analyses of two rabbit MAbs, R56 and R20, against the third variable region (V3) of HIV-1 gp120. R56 recognizes the well-studied immunogenic region in the V3 crown, while R20 targets a less-studied region at the C terminus of V3. By comparison of the Fab/epitope complex structures of these two antibodies raised by immunization with that of the corresponding human antibodies derived from patients chronically infected with HIV-1, we found that rabbit antibodies can recognize immunogenic regions of gp120 and mimic the binding modes of human antibodies. This result can provide new insight into the use of the rabbit as an animal model in AIDS vaccine development.

PubMed: 23864637
DOI: 10.1128/JVI.00843-13

実験手法

X-RAY DIFFRACTION (2.5 Å)