4JNB

Crystal structure of the Catalytic Domain of Human DUSP12

Summary for 4JNB

• Entry DOI: 10.2210/pdb4jnb/pdb
• Descriptor: Dual specificity protein phosphatase 12, SULFATE ION (2 entities in total)
• Functional Keywords: dusp, dual specificity phosphatase, phosphatase, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: Q9UNI6
• Total number of polymer chains: 1
• Total formula weight: 18715.30

Authors

Jeon, T.J.,Chien, P.N.,Ku, B.,Kim, S.J.,Ryu, S.E. (deposition date: 2013-03-15, release date: 2014-03-26, Last modification date: 2024-03-20)

Primary citation

Jeong, D.G.,Wei, C.H.,Ku, B.,Jeon, T.J.,Chien, P.N.,Kim, J.K.,Park, S.Y.,Hwang, H.S.,Ryu, S.Y.,Park, H.,Kim, D.S.,Kim, S.J.,Ryu, S.E.
The family-wide structure and function of human dual-specificity protein phosphatases.
Acta Crystallogr.,Sect.D, 70:421-435, 2014

PubMed Abstract: Dual-specificity protein phosphatases (DUSPs), which dephosphorylate both phosphoserine/threonine and phosphotyrosine, play vital roles in immune activation, brain function and cell-growth signalling. A family-wide structural library of human DUSPs was constructed based on experimental structure determination supplemented with homology modelling. The catalytic domain of each individual DUSP has characteristic features in the active site and in surface-charge distribution, indicating substrate-interaction specificity. The active-site loop-to-strand switch occurs in a subtype-specific manner, indicating that the switch process is necessary for characteristic substrate interactions in the corresponding DUSPs. A comprehensive analysis of the activity-inhibition profile and active-site geometry of DUSPs revealed a novel role of the active-pocket structure in the substrate specificity of DUSPs. A structure-based analysis of redox responses indicated that the additional cysteine residues are important for the protection of enzyme activity. The family-wide structures of DUSPs form a basis for the understanding of phosphorylation-mediated signal transduction and the development of therapeutics.

PubMed: 24531476
DOI: 10.1107/S1399004713029866

Experimental method

X-RAY DIFFRACTION (3 Å)