4JM4

Crystal Structure of PGT 135 Fab

Summary for 4JM4

• Entry DOI: 10.2210/pdb4jm4/pdb
• Related: 4JM2
• Descriptor: PGT 135 Heavy Chain, PGT 135 Light Chain (3 entities in total)
• Functional Keywords: immunoglobulin fold, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 49371.28

Authors

Kong, L.,Wilson, I.A. (deposition date: 2013-03-13, release date: 2013-05-29, Last modification date: 2024-10-16)

Primary citation

Kong, L.,Lee, J.H.,Doores, K.J.,Murin, C.D.,Julien, J.P.,McBride, R.,Liu, Y.,Marozsan, A.,Cupo, A.,Klasse, P.J.,Hoffenberg, S.,Caulfield, M.,King, C.R.,Hua, Y.,Le, K.M.,Khayat, R.,Deller, M.C.,Clayton, T.,Tien, H.,Feizi, T.,Sanders, R.W.,Paulson, J.C.,Moore, J.P.,Stanfield, R.L.,Burton, D.R.,Ward, A.B.,Wilson, I.A.
Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gp120.
Nat.Struct.Mol.Biol., 20:796-803, 2013

PubMed Abstract: A substantial proportion of the broadly neutralizing antibodies (bnAbs) identified in certain HIV-infected donors recognize glycan-dependent epitopes on HIV-1 gp120. Here we elucidate how the bnAb PGT 135 binds its Asn332 glycan-dependent epitope from its 3.1-Å crystal structure with gp120, CD4 and Fab 17b. PGT 135 interacts with glycans at Asn332, Asn392 and Asn386, using long CDR loops H1 and H3 to penetrate the glycan shield and access the gp120 protein surface. EM reveals that PGT 135 can accommodate the conformational and chemical diversity of gp120 glycans by altering its angle of engagement. Combined structural studies of PGT 135, PGT 128 and 2G12 show that this Asn332-dependent antigenic region is highly accessible and much more extensive than initially appreciated, which allows for multiple binding modes and varied angles of approach; thereby it represents a supersite of vulnerability for antibody neutralization.

PubMed: 23708606
DOI: 10.1038/nsmb.2594

Experimental method

X-RAY DIFFRACTION (1.751 Å)