4JKN

Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A

4JKN の概要

• エントリーDOI: 10.2210/pdb4jkn/pdb
• 関連するPDBエントリー: 3UGE
• 分子名称: Azurin, MERCURY (II) ION, NITRATE ION, ... (5 entities in total)
• 機能のキーワード: electron transport, mercury metallation
• 由来する生物種: Pseudomonas aeruginosa
• 細胞内の位置: Periplasm: P00282
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57380.24

構造登録者

Zampino, A.P.,Masters, F.M.,Bladholm, E.L.,Berry, S.B.,Panzner, M.J.,Ziegler, C.J. (登録日: 2013-03-10, 公開日: 2014-02-19, 最終更新日: 2024-11-27)

主引用文献

Zampino, A.P.,Masters, F.M.,Bladholm, E.L.,Panzner, M.J.,Berry, S.M.,Leeper, T.C.,Ziegler, C.J.
Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity.
J.Inorg.Biochem., 141:152-160, 2014

PubMed Abstract: Mercury(II) metallation of Pseudomonas aeruginosa azurin has been characterized structurally and biochemically. The X-ray crystal structure at 1.5Å of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings are further validated by NMR, Matrix-assisted laser desorption/ionization spectrometry (MALDI), and UV-visible spectroscopic methods indicating copper displacement from the wild-type protein. Bioinformatic analysis has identified homologous human protein domains computationally, and compared them to the structure of azurin, providing a model for human mercury interactions. Study of the mercury-azurin adduct, in combination with other known examples of protein-heavy metal interactions, could provide further insight into the chemical mechanisms of toxicological interactions, leading toward a global understanding of the biological speciation of toxic heavy metals.

PubMed: 25265377
DOI: 10.1016/j.jinorgbio.2014.09.003

実験手法

X-RAY DIFFRACTION (1.536 Å)