4JJ3

Crystal structure of MamP soaked with iron(II)

4JJ3 の概要

• エントリーDOI: 10.2210/pdb4jj3/pdb
• 関連するPDBエントリー: 4JJ0
• 分子名称: MamP, HEME C (2 entities in total)
• 機能のキーワード: pdz, cytochrome, magnetochrome, electron transport
• 由来する生物種: Magnetococcus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54786.67

構造登録者

Siponen, M.,Pignol, D.,Arnoux, P. (登録日: 2013-03-07, 公開日: 2013-10-09, 最終更新日: 2024-12-25)

主引用文献

Siponen, M.I.,Legrand, P.,Widdrat, M.,Jones, S.R.,Zhang, W.J.,Chang, M.C.,Faivre, D.,Arnoux, P.,Pignol, D.
Structural insight into magnetochrome-mediated magnetite biomineralization.
Nature, 502:681-684, 2013

PubMed Abstract: Magnetotactic bacteria align along the Earth's magnetic field using an organelle called the magnetosome, a biomineralized magnetite (Fe(II)Fe(III)2O4) or greigite (Fe(II)Fe(III)2S4) crystal embedded in a lipid vesicle. Although the need for both iron(II) and iron(III) is clear, little is known about the biological mechanisms controlling their ratio. Here we present the structure of the magnetosome-associated protein MamP and find that it is built on a unique arrangement of a self-plugged PDZ domain fused to two magnetochrome domains, defining a new class of c-type cytochrome exclusively found in magnetotactic bacteria. Mutational analysis, enzyme kinetics, co-crystallization with iron(II) and an in vitro MamP-assisted magnetite production assay establish MamP as an iron oxidase that contributes to the formation of iron(III) ferrihydrite eventually required for magnetite crystal growth in vivo. These results demonstrate the molecular mechanisms of iron management taking place inside the magnetosome and highlight the role of magnetochrome in iron biomineralization.

PubMed: 24097349
DOI: 10.1038/nature12573

実験手法

X-RAY DIFFRACTION (2.8 Å)