4JIZ

Human Mob1-phosphopeptide complex

Summary for 4JIZ

• Entry DOI: 10.2210/pdb4jiz/pdb
• Descriptor: MOB kinase activator 1A, phosphopeptide, ZINC ION, ... (4 entities in total)
• Functional Keywords: signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 21377.72

Authors

Stach, L.,Ogrodowicz, R.W.,Rock, J.M.,Lim, D.,Yaffe, M.B.,Amon, A.,Smerdon, S.J. (deposition date: 2013-03-07, release date: 2013-04-17, Last modification date: 2024-11-06)

Primary citation

Rock, J.M.,Lim, D.,Stach, L.,Ogrodowicz, R.W.,Keck, J.M.,Jones, M.H.,Wong, C.C.,Yates, J.R.,Winey, M.,Smerdon, S.J.,Yaffe, M.B.,Amon, A.
Activation of the yeast Hippo pathway by phosphorylation-dependent assembly of signaling complexes.
Science, 340:871-875, 2013

PubMed Abstract: Scaffold-assisted signaling cascades guide cellular decision-making. In budding yeast, one such signal transduction pathway called the mitotic exit network (MEN) governs the transition from mitosis to the G1 phase of the cell cycle. The MEN is conserved and in metazoans is known as the Hippo tumor-suppressor pathway. We found that signaling through the MEN kinase cascade was mediated by an unusual two-step process. The MEN kinase Cdc15 first phosphorylated the scaffold Nud1. This created a phospho-docking site on Nud1, to which the effector kinase complex Dbf2-Mob1 bound through a phosphoserine-threonine binding domain, in order to be activated by Cdc15. This mechanism of pathway activation has implications for signal transmission through other kinase cascades and might represent a general principle in scaffold-assisted signaling.

PubMed: 23579499
DOI: 10.1126/science.1235822

Experimental method

X-RAY DIFFRACTION (2.1 Å)