4JIF

Co-crystal structure of ICAP1 PTB domain in complex with a KRIT1 peptide

Summary for 4JIF

• Entry DOI: 10.2210/pdb4jif/pdb
• Descriptor: Integrin beta-1-binding protein 1, Krev interaction trapped protein 1 (3 entities in total)
• Functional Keywords: ptb fold, integrin signaling, integrin binding, signaling protein
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: O14713; Cytoplasm, cytoskeleton: O00522
• Total number of polymer chains: 2
• Total formula weight: 20635.28

Authors

Liu, W.,Boggon, T.J. (deposition date: 2013-03-05, release date: 2013-05-01, Last modification date: 2023-09-20)

Primary citation

Liu, W.,Boggon, T.J.
Cocrystal structure of the ICAP1 PTB domain in complex with a KRIT1 peptide.
Acta Crystallogr.,Sect.F, 69:494-498, 2013

PubMed Abstract: Integrin cytoplasmic domain-associated protein-1 (ICAP1) is a suppressor of integrin activation and directly binds to the cytoplasmic tail of β1 integrins; its binding suppresses integrin activation by competition with talin. Krev/Rap1 interaction trapped-1 (KRIT1) releases ICAP1 suppression of integrin activation by sequestering ICAP1 away from integrin cytoplasmic tails. Here, the cocrystal structure of the PTB domain of ICAP1 in complex with a 29-amino-acid fragment (residues 170-198) of KRIT1 is presented to 1.7 Å resolution [the resolution at which 〈I/σ(I)〉 = 2.9 was 1.83 Å]. In previous studies, the structure of ICAP1 with integrin β1 was determined to 3.0 Å resolution and that of ICAP1 with the N-terminal portion of KRIT1 (residues 1-198) was determined to 2.54 Å resolution; therefore, this study provides the highest resolution structure yet of ICAP1 and allows further detailed analysis of the interaction of ICAP1 with its minimal binding region in KRIT1.

PubMed: 23695561
DOI: 10.1107/S1744309113010762

Experimental method

X-RAY DIFFRACTION (1.701 Å)