4JIF

Co-crystal structure of ICAP1 PTB domain in complex with a KRIT1 peptide

4JIF の概要

• エントリーDOI: 10.2210/pdb4jif/pdb
• 分子名称: Integrin beta-1-binding protein 1, Krev interaction trapped protein 1 (3 entities in total)
• 機能のキーワード: ptb fold, integrin signaling, integrin binding, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: O14713; Cytoplasm, cytoskeleton: O00522
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20635.28

構造登録者

Liu, W.,Boggon, T.J. (登録日: 2013-03-05, 公開日: 2013-05-01, 最終更新日: 2023-09-20)

主引用文献

Liu, W.,Boggon, T.J.
Cocrystal structure of the ICAP1 PTB domain in complex with a KRIT1 peptide.
Acta Crystallogr.,Sect.F, 69:494-498, 2013

PubMed Abstract: Integrin cytoplasmic domain-associated protein-1 (ICAP1) is a suppressor of integrin activation and directly binds to the cytoplasmic tail of β1 integrins; its binding suppresses integrin activation by competition with talin. Krev/Rap1 interaction trapped-1 (KRIT1) releases ICAP1 suppression of integrin activation by sequestering ICAP1 away from integrin cytoplasmic tails. Here, the cocrystal structure of the PTB domain of ICAP1 in complex with a 29-amino-acid fragment (residues 170-198) of KRIT1 is presented to 1.7 Å resolution [the resolution at which 〈I/σ(I)〉 = 2.9 was 1.83 Å]. In previous studies, the structure of ICAP1 with integrin β1 was determined to 3.0 Å resolution and that of ICAP1 with the N-terminal portion of KRIT1 (residues 1-198) was determined to 2.54 Å resolution; therefore, this study provides the highest resolution structure yet of ICAP1 and allows further detailed analysis of the interaction of ICAP1 with its minimal binding region in KRIT1.

PubMed: 23695561
DOI: 10.1107/S1744309113010762

実験手法

X-RAY DIFFRACTION (1.701 Å)