4JEK

Structure of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis

Summary for 4JEK

• Entry DOI: 10.2210/pdb4jek/pdb
• Descriptor: Dibenzothiophene desulfurization enzyme C (2 entities in total)
• Functional Keywords: dibenzothiophene desulfurization enzyme c, oxidoreductase
• Biological source: Rhodococcus sp.
• Total number of polymer chains: 8
• Total formula weight: 391232.94

Authors

Zhang, L.,Duan, X.L.,Zhou, D.M.,Li, X. (deposition date: 2013-02-27, release date: 2013-09-18, Last modification date: 2024-03-20)

Primary citation

Duan, X.,Zhang, L.,Zhou, D.,Ji, K.,Ma, T.,Shui, W.,Li, G.,Li, X.
Crystallization and preliminary structural analysis of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis
Acta Crystallogr.,Sect.F, 69:597-601, 2013

PubMed Abstract: Dibenzothiophene (DBT) and its derivatives are typical sulfur compounds found in fossil fuels. These compounds show resistance to the hydrodesulfurization treatment that is commonly used in industry. Dibenzothiophene monooxygenase (DszC) is responsible for the oxidation of DBT, which is the first and the rate-limiting step in the DBT enzymatic desulfurization 4S pathway. In this study, the crystal structure of DszC from Rhodococcus erythropolis DS-3 is reported. The crystal of native DszC belonged to space group P1, with unit-cell parameters a = 96.16, b = 96.27, c = 98.56 Å, α = 81.03, β = 67.57, γ = 85.84°. To determine the phase, SAD X-ray diffraction data were collected from a SeMet-derivative DszC crystal, which also belonged to space group P1, with unit-cell parameters a = 95.379, b = 95.167, c = 94.891 Å, α = 87.046, β = 70.536, γ = 79.738°. Further structural analysis of DszC is in progress.

PubMed: 23722833
DOI: 10.1107/S1744309113011172

Experimental method

X-RAY DIFFRACTION (2.4 Å)