4JDY

Crystal structure of Rv2606c

4JDY の概要

• エントリーDOI: 10.2210/pdb4jdy/pdb
• 関連するPDBエントリー: 2iss 2zbt
• 分子名称: Pyridoxal biosynthesis lyase PdxS, GLYCEROL (3 entities in total)
• 機能のキーワード: (beta/alpha)8-barrel, lyase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 83835.50

構造登録者

Kim, S.,Kim, K.-J. (登録日: 2013-02-25, 公開日: 2013-05-22, 最終更新日: 2023-11-08)

主引用文献

Kim, S.,Kim, K.J.
Crystal structure of Mycobacterium tuberculosis Rv2606c: a pyridoxal biosynthesis lyase.
Biochem.Biophys.Res.Commun., 435:255-259, 2013

PubMed Abstract: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. We determined the crystal structure of Rv2606c, a potential pyridoxal biosynthesis lyase (PdxS), from M. tuberculosis H37Rv at 1.8 Å resolution. The overall structure of the protein, composed of a (β/α)8-barrel and two small 310-helices, was quite similar to those of other PdxS proteins. A glycerol molecule was observed to be bound at the active site of the Rv2606c structure through interactions with the conserved residues of Asp29 and Lys86, providing information regarding the potential active site and the substrate-binding environment of the protein. The interface for Rv2606c dodecamerization, which is primarily mediated by salt bridges and hydrophobic interactions, was quite different from those of other PdxS proteins. Furthermore, we observed that the Rv2606c and Rv2604c form a stable complex, suggesting that these proteins might function as PdxS and PdxT in M. tuberculosis.

PubMed: 23643787
DOI: 10.1016/j.bbrc.2013.04.068

実験手法

X-RAY DIFFRACTION (1.8 Å)