4JD2

Crystal structure of Bos taurus Arp2/3 complex binding with Mus musculus GMF

4JD2 の概要

• エントリーDOI: 10.2210/pdb4jd2/pdb
• 分子名称: Actin-related protein 3, ADENOSINE-5'-TRIPHOSPHATE, TETRAETHYLENE GLYCOL, ... (11 entities in total)
• 機能のキーワード: actin filament polymerization and branching, structural protein
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): P61157 A7MB62 Q58CQ2 Q3MHR7 Q3T035 Q148J6 Q3SYX9
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 242454.87

構造登録者

Nolen, B.J.,Luan, Q. (登録日: 2013-02-22, 公開日: 2013-07-17, 最終更新日: 2024-02-28)

主引用文献

Luan, Q.,Nolen, B.J.
Structural basis for regulation of Arp2/3 complex by GMF.
Nat.Struct.Mol.Biol., 20:1062-1068, 2013

PubMed Abstract: The Arp2/3 complex mediates formation of complex cellular structures such as lamellipodia by nucleating branched actin filaments. Arp2/3-complex activity is precisely controlled by over a dozen regulators, yet the structural mechanism by which regulators interact with the complex is unknown. GMF is a recently discovered regulator of the Arp2/3 complex that can inhibit nucleation and disassemble branches. We solved the structure of the 240-kDa assembly of Mus musculus GMF and Bos taurus Arp2/3 complex and found that GMF binds the barbed end of Arp2, overlapping with the proposed binding site of WASP-family proteins. The structure suggests that GMF can bind branch junctions in the manner that cofilin binds filament sides, consistent with a modified cofilin-like mechanism for debranching by GMF. The GMF-Arp2 interface reveals how the ADF-H actin-binding domain in GMF is exploited to specifically recognize Arp2/3 complex and not actin.

PubMed: 23893131
DOI: 10.1038/nsmb.2628

実験手法

X-RAY DIFFRACTION (3.08 Å)