4J90

Square-shaped octameric structure of KtrA with ATP bound

4J90 の概要

• エントリーDOI: 10.2210/pdb4j90/pdb
• 関連するPDBエントリー: 2HMS 2HMT 2HMU 2HMV 2HMW 4J91
• 分子名称: Ktr system potassium uptake protein A, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• 機能のキーワード: square-shaped octameric ring, rck domain, potassium transport regulation, ktrb membrane protein, cytosol, metal transport
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): O32080
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50847.88

構造登録者

Vieira-Pires, R.S.,Morais-Cabral, J.H. (登録日: 2013-02-15, 公開日: 2013-04-17, 最終更新日: 2024-03-20)

主引用文献

Vieira-Pires, R.S.,Szollosi, A.,Morais-Cabral, J.H.
The structure of the KtrAB potassium transporter
Nature, 496:323-328, 2013

PubMed Abstract: In bacteria, archaea, fungi and plants the Trk, Ktr and HKT ion transporters are key components of osmotic regulation, pH homeostasis and resistance to drought and high salinity. These ion transporters are functionally diverse: they can function as Na(+) or K(+) channels and possibly as cation/K(+) symporters. They are closely related to potassium channels both at the level of the membrane protein and at the level of the cytosolic regulatory domains. Here we describe the crystal structure of a Ktr K(+) transporter, the KtrAB complex from Bacillus subtilis. The structure shows the dimeric membrane protein KtrB assembled with a cytosolic octameric KtrA ring bound to ATP, an activating ligand. A comparison between the structure of KtrAB-ATP and the structures of the isolated full-length KtrA protein with ATP or ADP reveals a ligand-dependent conformational change in the octameric ring, raising new ideas about the mechanism of activation in these transporters.

PubMed: 23598340
DOI: 10.1038/nature12055

実験手法

X-RAY DIFFRACTION (3.24 Å)