4J4M
Crystal structure of TM-1, a Trimeresurus mucrosquamatus venom metalloproteinase
4J4M の概要
エントリーDOI | 10.2210/pdb4j4m/pdb |
関連するPDBエントリー | 1KUF |
分子名称 | zinc-dependent metalloproteinase, ZINC ION (3 entities in total) |
機能のキーワード | alpha/beta-mixed fold, endopeptidase, hydrolase |
由来する生物種 | Protobothrops mucrosquamatus (Taiwan habu) |
タンパク質・核酸の鎖数 | 2 |
化学式量合計 | 44011.74 |
構造登録者 | |
主引用文献 | Chou, T.L.,Wu, C.H.,Huang, K.F.,Wang, A.H. Crystal structure of a Trimeresurus mucrosquamatus venom metalloproteinase providing new insights into the inhibition by endogenous tripeptide inhibitors. Toxicon, 71C:140-146, 2013 Cited by PubMed Abstract: The crystal structure of TM-1, a P-I class snake-venom metalloproteinase (SVMP) from the Trimeresurus mucrosquamatus venom, was determined at 1.8-Å resolution. The structure exhibits the typical feature of SVMPs and is stabilized by three disulfide linkages. The active site shows a deep S1' substrate-binding pocket limited by the non-conserved Pro174 at the bottom. Further comparisons with other SVMPs suggest that the deep S1' site of TM-1 correlates with its high inhibition sensitivity to the endogenous tripeptide inhibitors. Proteolytic specificity analysis revealed that TM-1 prefers substrates having a moderate-size and hydrophobic residue at the P1' position, consistent with our structural observation. PubMed: 23732127DOI: 10.1016/j.toxicon.2013.05.009 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (1.8 Å) |
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