4J1S

Crystal structure of a ketoreductase domain from the bacillaene assembly line

4J1S の概要

• エントリーDOI: 10.2210/pdb4j1s/pdb
• 関連するPDBエントリー: 4J1Q
• 分子名称: Polyketide synthase PksJ (1 entity in total)
• 機能のキーワード: rossmann fold, ketoreductase, oxidoreductase
• 由来する生物種: Bacillus subtilis subsp. subtilis
• 細胞内の位置: Cytoplasm: P40806
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52515.57

構造登録者

Zheng, J.,Keatinge-Clay, A.T. (登録日: 2013-02-01, 公開日: 2014-03-19, 最終更新日: 2023-09-20)

主引用文献

Piasecki, S.K.,Zheng, J.,Axelrod, A.J.,E Detelich, M.,Keatinge-Clay, A.T.
Structural and functional studies of a trans-acyltransferase polyketide assembly line enzyme that catalyzes stereoselective alpha- and beta-ketoreduction.
Proteins, 82:2067-2077, 2014

PubMed Abstract: While the cis-acyltransferase modular polyketide synthase assembly lines have largely been structurally dissected, enzymes from within the recently discovered trans-acyltransferase polyketide synthase assembly lines are just starting to be observed crystallographically. Here we examine the ketoreductase (KR) from the first polyketide synthase module of the bacillaene nonribosomal peptide synthetase/polyketide synthase at 2.35-Å resolution. This KR naturally reduces both α- and β-keto groups and is the only KR known to do so during the biosynthesis of a polyketide. The isolated KR not only reduced an N-acetylcysteamine-bound β-keto substrate to a D-β-hydroxy product, but also an N-acetylcysteamine-bound α-keto substrate to an L-α-hydroxy product. That the substrates must enter the active site from opposite directions to generate these stereochemistries suggests that the acyl-phosphopantetheine moiety is capable of accessing very different conformations despite being anchored to a serine residue of a docked acyl carrier protein. The features enabling stereocontrolled α-ketoreduction may not be extensive since a KR that naturally reduces a β-keto group within a cis-acyltransferase polyketide synthase was identified that performs a completely stereoselective reduction of the same α-keto substrate to generate the D-α-hydroxy product. A sequence analysis of trans-acyltransferase KRs reveals that a single residue, rather than a three-residue motif found in cis-acyltransferase KRs, is predictive of the orientation of the resulting β-hydroxyl group.

PubMed: 24634061
DOI: 10.1002/prot.24561

実験手法

X-RAY DIFFRACTION (3.01 Å)