4J1L

Mutant Endotoxin TeNT

Summary for 4J1L

• Entry DOI: 10.2210/pdb4j1l/pdb
• Descriptor: Tetanus toxin, ZINC ION (3 entities in total)
• Functional Keywords: endotoxin, toxin
• Biological source: Clostridium tetani
• Total number of polymer chains: 1
• Total formula weight: 49128.53

Authors

Guo, J.,Pan, X.,Zhao, Y.,Chen, S. (deposition date: 2013-02-01, release date: 2014-02-05, Last modification date: 2024-03-20)

Primary citation

Guo, J.,Pan, X.,Zhao, Y.,Chen, S.
Engineering Clostridia Neurotoxins with elevated catalytic activity
Toxicon, 74:158-166, 2013

PubMed Abstract: BoNT/B and TeNT cleave substrate VAMP2 at the same scissile bond, yet these two toxins showed different efficiency on substrate hydrolysis and had different requirements for the recognition of P2' site of VAMP2, E(78). These differences may be due to their different composition of their substrate recognition pockets in the active site. Swapping of LC/T S1' pocket residue, L(230), with the corresponding isoleucine in LC/B increased LC/T activity by ∼25 fold, while swapping of LC/B S1' pocket residue, S(201), with the corresponding proline in LC/T increased LC/B activity by ∼10 fold. Optimization of both S1 and S1' pocket residues of LC/T, LC/T (K(168)E, L(230)I) elevated LC/T activity by more than 100-fold. The highly active LC/T derivative engineered in this study has the potential to be used as a more effective tool to study mechanisms of exocytosis in central neuron. The LC/B derivative with elevated activity has the potential to be developed into novel therapy to minimize the impact of immunoresistance during BoNT/B therapy.

PubMed: 23994593
DOI: 10.1016/j.toxicon.2013.08.055

Experimental method

X-RAY DIFFRACTION (2.6 Å)