4J0L

Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules

Summary for 4J0L

• Entry DOI: 10.2210/pdb4j0l/pdb
• Descriptor: Basic endochitinase C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: chitinase, hydrolase, carbohydrate
• Biological source: Secale cereale (Rye)
• Total number of polymer chains: 1
• Total formula weight: 28343.13

Authors

Umemoto, N.,Numata, T.,Ohnuma, T.,Fukamizo, T. (deposition date: 2013-01-31, release date: 2013-10-16, Last modification date: 2024-10-30)

Primary citation

Ohnuma, T.,Umemoto, N.,Kondo, K.,Numata, T.,Fukamizo, T.
Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure
Febs Lett., 587:2691-2697, 2013

PubMed Abstract: Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)₄ molecules. One (GlcNAc)₄ molecule bound to subsites -4 to -1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase.

PubMed: 23871710
DOI: 10.1016/j.febslet.2013.07.008

Experimental method

X-RAY DIFFRACTION (1.9 Å)