4J0L

Crystal Structure of a Family GH19 Chitinase (W72A/E67Q mutant) from rye seeds in complex with two (GlcNAc)4 molecules

4J0L の概要

• エントリーDOI: 10.2210/pdb4j0l/pdb
• 分子名称: Basic endochitinase C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: chitinase, hydrolase, carbohydrate
• 由来する生物種: Secale cereale (Rye)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28343.13

構造登録者

Umemoto, N.,Numata, T.,Ohnuma, T.,Fukamizo, T. (登録日: 2013-01-31, 公開日: 2013-10-16, 最終更新日: 2024-10-30)

主引用文献

Ohnuma, T.,Umemoto, N.,Kondo, K.,Numata, T.,Fukamizo, T.
Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure
Febs Lett., 587:2691-2697, 2013

PubMed Abstract: Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely occupied with the sugar residues of two (GlcNAc)₄ molecules. One (GlcNAc)₄ molecule bound to subsites -4 to -1, while the other bound to subsites +1 to +4. Comparisons of the main chain conformation between liganded RSC-c-E67Q/W72A and unliganded wild type RSC-c suggested domain motion essential for catalysis. This is the first report on the complete subsite mapping of GH19 chitinase.

PubMed: 23871710
DOI: 10.1016/j.febslet.2013.07.008

実験手法

X-RAY DIFFRACTION (1.9 Å)