4IXQ

RT fs X-ray diffraction of Photosystem II, dark state

4IXQ の概要

• エントリーDOI: 10.2210/pdb4ixq/pdb
• 関連するPDBエントリー: 4IXR
• 分子名称: Photosystem Q(B) protein 1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (35 entities in total)
• 機能のキーワード: iron, manganese, ps ii, ps2, transmembrane alpha-helix, electron transport, reaction center, water oxidation, membrane complex, thylakoid membrane, photosynthesis
• 由来する生物種: Thermosynechococcus elongatus; 詳細
• 細胞内の位置: Cellular thylakoid membrane; Multi-pass membrane protein: P0A444 Q9F1R6; Cellular thylakoid membrane; Single-pass membrane protein: Q9F1K9 Q8DIN8 Q8DHA7 Q8DIQ0 Q8DHJ2 Q8DIP0 Q8DIN9 Q8DJ43 Q8DJZ6; Cellular thylakoid membrane; Peripheral membrane protein; Lumenal side: Q9F1L5 P0A386; Cellular thylakoid membrane; Single-pass membrane protein (By similarity): Q8DJI1 P59087; Cellular thylakoid membrane; Multi-pass membrane protein (By similarity): Q8CM25
• タンパク質・核酸の鎖数: 40
• 化学式量合計: 765686.00

構造登録者

Kern, J.,Alonso-Mori, R.,Tran, R.,Hattne, J.,Gildea, R.J.,Echols, N.,Gloeckner, C.,Hellmich, J.,Laksmono, H.,Sierra, R.G.,Lassalle-Kaiser, B.,Koroidov, S.,Lampe, A.,Han, G.,Gul, S.,DiFiore, D.,Milathianaki, D.,Fry, A.R.,Miahnahri, A.,Schafer, D.W.,Messerschmidt, M.,Seibert, M.M.,Koglin, J.E.,Sokaras, D.,Weng, T.-C.,Sellberg, J.,Latimer, M.J.,Grosse-Kunstleve, R.W.,Zwart, P.H.,White, W.E.,Glatzel, P.,Adams, P.D.,Bogan, M.J.,Williams, G.J.,Boutet, S.,Messinger, J.,Zouni, A.,Sauter, N.K.,Yachandra, V.K.,Bergmann, U.,Yano, J. (登録日: 2013-01-27, 公開日: 2013-02-20, 最終更新日: 2024-10-30)

主引用文献

Kern, J.,Alonso-Mori, R.,Tran, R.,Hattne, J.,Gildea, R.J.,Echols, N.,Glockner, C.,Hellmich, J.,Laksmono, H.,Sierra, R.G.,Lassalle-Kaiser, B.,Koroidov, S.,Lampe, A.,Han, G.,Gul, S.,Difiore, D.,Milathianaki, D.,Fry, A.R.,Miahnahri, A.,Schafer, D.W.,Messerschmidt, M.,Seibert, M.M.,Koglin, J.E.,Sokaras, D.,Weng, T.C.,Sellberg, J.,Latimer, M.J.,Grosse-Kunstleve, R.W.,Zwart, P.H.,White, W.E.,Glatzel, P.,Adams, P.D.,Bogan, M.J.,Williams, G.J.,Boutet, S.,Messinger, J.,Zouni, A.,Sauter, N.K.,Yachandra, V.K.,Bergmann, U.,Yano, J.
Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature.
Science, 340:491-495, 2013

PubMed Abstract: Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.

PubMed: 23413188
DOI: 10.1126/science.1234273

実験手法

X-RAY DIFFRACTION (5.7 Å)